pCDNA4/Full length FVIII

LOCUS       pCDNA4/Full length FVIII               12086 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCDNA4/Full length FVIII
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Peters RT, Toby G, Lu Q, Liu T, Kulman JD, Low SC, Bitonti AJ, 
            Pierce GF
  TITLE     Biochemical and functional characterization of a recombinant 
            monomeric Factor VIII-Fc fusion protein.
  JOURNAL   J Thromb Haemost. 2012 Dec 4. doi: 10.1111/jth.12076.
  PUBMED    23205847
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCDNA4/Full length FVIII|
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             7143..7172
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7188..7205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7228..7245)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7234..7458
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7504..7932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7591..7610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7801..7822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7941..7961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7946..8276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8127..8262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8189..8208
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8324..8371
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8390..8764
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8894..9015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8931..8950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8985..9004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9064..9080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9064..9080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9077..9099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9088..9104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9112..9142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9157..9178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9295..9312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9466..10054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9546..9565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10225..11085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10848..10867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11086..11190)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11265..11284)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11456..11835
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11836..12039
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11990..12010
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12084..17
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12084..16
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tacgactcac tatagggaga cccaagctgg ctagttaagc ttcgcgacgt acggccgcca
       61 ccatgcaaat agagctctcc acctgcttct ttctgtgcct tttgcgattc tgctttagtg
      121 ccaccagaag atactacctg ggtgcagtgg aactgtcatg ggactatatg caaagtgatc
      181 tcggtgagct gcctgtggac gcaagatttc ctcctagagt gccaaaatct tttccattca
      241 acacctcagt cgtgtacaaa aagactctgt ttgtagaatt cacggatcac cttttcaaca
      301 tcgctaagcc aaggccaccc tggatgggtc tgctaggtcc taccatccag gctgaggttt
      361 atgatacagt ggtcattaca cttaagaaca tggcttccca tcctgtcagt cttcatgctg
      421 ttggtgtatc ctactggaaa gcttctgagg gagctgaata tgatgatcag accagtcaaa
      481 gggagaaaga agatgataaa gtcttccctg gtggaagcca tacatatgtc tggcaggtcc
      541 tgaaagagaa tggtccaatg gcctctgacc cactgtgcct tacctactca tatctttctc
      601 atgtggacct ggtaaaagac ttgaattcag gcctcattgg agccctacta gtatgtagag
      661 aagggagtct ggccaaggaa aagacacaga ccttgcacaa atttatacta ctttttgctg
      721 tatttgatga agggaaaagt tggcactcag aaacaaagaa ctccttgatg caggataggg
      781 atgctgcatc tgctcgggcc tggcctaaaa tgcacacagt caatggttat gtaaacaggt
      841 ctctgccagg tctgattgga tgccacagga aatcagtcta ttggcatgtg attggaatgg
      901 gcaccactcc tgaagtgcac tcaatattcc tcgaaggtca cacatttctt gtgaggaacc
      961 atcgccaggc gtccttggaa atctcgccaa taactttcct tactgctcaa acactcttga
     1021 tggaccttgg acagtttcta ctgttttgtc atatctcttc ccaccaacat gatggcatgg
     1081 aagcttatgt caaagtagac agctgtccag aggaacccca actacgaatg aaaaataatg
     1141 aagaagcgga agactatgat gatgatctta ctgattctga aatggatgtg gtcaggtttg
     1201 atgatgacaa ctctccttcc tttatccaaa ttcgctcagt tgccaagaag catcctaaaa
     1261 cttgggtaca ttacattgct gctgaagagg aggactggga ctatgctccc ttagtcctcg
     1321 cccccgatga cagaagttat aaaagtcaat atttgaacaa tggccctcag cggattggta
     1381 ggaagtacaa aaaagtccga tttatggcat acacagatga aacctttaag actcgtgaag
     1441 ctattcagca tgaatcagga atcttgggac ctttacttta tggggaagtt ggagacacac
     1501 tgttgattat atttaagaat caagcaagca gaccatataa catctaccct cacggaatca
     1561 ctgatgtccg tcctttgtat tcaaggagat taccaaaagg tgtaaaacat ttgaaggatt
     1621 ttccaattct gccaggagaa atattcaaat ataaatggac agtgactgta gaagatgggc
     1681 caactaaatc agatcctcgg tgcctgaccc gctattactc tagtttcgtt aatatggaga
     1741 gagatctagc ttcaggactc attggccctc tcctcatctg ctacaaagaa tctgtagatc
     1801 aaagaggaaa ccagataatg tcagacaaga ggaatgtcat cctgttttct gtatttgatg
     1861 agaaccgaag ctggtacctc acagagaata tacaacgctt tctccccaat ccagctggag
     1921 tgcagcttga ggatccagag ttccaagcct ccaacatcat gcacagcatc aatggctatg
     1981 tttttgatag tttgcagttg tcagtttgtt tgcatgaggt ggcatactgg tacattctaa
     2041 gcattggagc acagactgac ttcctttctg tcttcttctc tggatatacc ttcaaacaca
     2101 aaatggtcta tgaagacaca ctcaccctat tcccattctc aggagaaact gtcttcatgt
     2161 cgatggaaaa cccaggtcta tggattctgg ggtgccacaa ctcagacttt cggaacagag
     2221 gcatgaccgc cttactgaag gtttctagtt gtgacaagaa cactggtgat tattacgagg
     2281 acagttatga agatatttca gcatacttgc tgagtaaaaa caatgccatt gaaccaagaa
     2341 gcttctccca gaattcaaga caccctagca ctaggcaaaa gcaatttaat gccaccacaa
     2401 ttccagaaaa tgacatagag aagactgacc cttggtttgc acacagaaca cctatgccta
     2461 aaatacaaaa tgtctcctct agtgatttgt tgatgctctt gcgacagagt cctactccac
     2521 atgggctatc cttatctgat ctccaagaag ccaaatatga gactttttct gatgatccat
     2581 cacctggagc aatagacagt aataacagcc tgtctgaaat gacacacttc aggccacagc
     2641 tccatcacag tggggacatg gtatttaccc ctgagtcagg cctccaatta agattaaatg
     2701 agaaactggg gacaactgca gcaacagagt tgaagaaact tgatttcaaa gtttctagta
     2761 catcaaataa tctgatttca acaattccat cagacaattt ggcagcaggt actgataata
     2821 caagttcctt aggaccccca agtatgccag ttcattatga tagtcaatta gataccactc
     2881 tatttggcaa aaagtcatct ccccttactg agtctggtgg acctctgagc ttgagtgaag
     2941 aaaataatga ttcaaagttg ttagaatcag gtttaatgaa tagccaagaa agttcatggg
     3001 gaaaaaatgt atcgtcaaca gagagtggta ggttatttaa agggaaaaga gctcatggac
     3061 ctgctttgtt gactaaagat aatgccttat tcaaagttag catctctttg ttaaagacaa
     3121 acaaaacttc caataattca gcaactaata gaaagactca cattgatggc ccatcattat
     3181 taattgagaa tagtccatca gtctggcaaa atatattaga aagtgacact gagtttaaaa
     3241 aagtgacacc tttgattcat gacagaatgc ttatggacaa aaatgctaca gctttgaggc
     3301 taaatcatat gtcaaataaa actacttcat caaaaaacat ggaaatggtc caacagaaaa
     3361 aagagggccc cattccacca gatgcacaaa atccagatat gtcgttcttt aagatgctat
     3421 tcttgccaga atcagcaagg tggatacaaa ggactcatgg aaagaactct ctgaactctg
     3481 ggcaaggccc cagtccaaag caattagtat ccttaggacc agaaaaatct gtggaaggtc
     3541 agaatttctt gtctgagaaa aacaaagtgg tagtaggaaa gggtgaattt acaaaggacg
     3601 taggactcaa agagatggtt tttccaagca gcagaaacct atttcttact aacttggata
     3661 atttacatga aaataataca cacaatcaag aaaaaaaaat tcaggaagaa atagaaaaga
     3721 aggaaacatt aatccaagag aatgtagttt tgcctcagat acatacagtg actggcacta
     3781 agaatttcat gaagaacctt ttcttactga gcactaggca aaatgtagaa ggttcatatg
     3841 acggggcata tgctccagta cttcaagatt ttaggtcatt aaatgattca acaaatagaa
     3901 caaagaaaca cacagctcat ttctcaaaaa aaggggagga agaaaacttg gaaggcttgg
     3961 gaaatcaaac caagcaaatt gtagagaaat atgcatgcac cacaaggata tctcctaata
     4021 caagccagca gaattttgtc acgcaacgta gtaagagagc tttgaaacaa ttcagactcc
     4081 cactagaaga aacagaactt gaaaaaagga taattgtgga tgacacctca acccagtggt
     4141 ccaaaaacat gaaacatttg accccgagca ccctcacaca gatagactac aatgagaagg
     4201 agaaaggggc cattactcag tctcccttat cagattgcct tacgaggagt catagcatcc
     4261 ctcaagcaaa tagatctcca ttacccattg caaaggtatc atcatttcca tctattagac
     4321 ctatatatct gaccagggtc ctattccaag acaactcttc tcatcttcca gcagcatctt
     4381 atagaaagaa agattctggg gtccaagaaa gcagtcattt cttacaagga gccaaaaaaa
     4441 ataacctttc tttagccatt ctaaccttgg agatgactgg tgatcaaaga gaggttggct
     4501 ccctggggac aagtgccaca aattcagtca catacaagaa agttgagaac actgttctcc
     4561 cgaaaccaga cttgcccaaa acatctggca aagttgaatt gcttccaaaa gttcacattt
     4621 atcagaagga cctattccct acggaaacta gcaatgggtc tcctggccat ctggatctcg
     4681 tggaagggag ccttcttcag ggaacagagg gagcgattaa gtggaatgaa gcaaacagac
     4741 ctggaaaagt tccctttctg agagtagcaa cagaaagctc tgcaaagact ccctccaagc
     4801 tattggatcc tcttgcttgg gataaccact atggtactca gataccaaaa gaagagtgga
     4861 aatcccaaga gaagtcacca gaaaaaacag cttttaagaa aaaggatacc attttgtccc
     4921 tgaacgcttg tgaaagcaat catgcaatag cagcaataaa tgagggacaa aataagcccg
     4981 aaatagaagt cacctgggca aagcaaggta ggactgaaag gctgtgctct caaaacccac
     5041 cagtcttgaa acgccatcaa cgggaaataa ctcgtactac tcttcagtca gatcaagagg
     5101 aaattgacta tgatgatacc atatcagttg aaatgaagaa ggaagatttt gacatttatg
     5161 atgaggatga aaatcagagc ccccgcagct ttcaaaagaa aacacgacac tattttattg
     5221 ctgcagtgga gaggctctgg gattatggga tgagtagctc cccacatgtt ctaagaaaca
     5281 gggctcagag tggcagtgtc cctcagttca agaaagttgt tttccaggaa tttactgatg
     5341 gctcctttac tcagccctta taccgtggag aactaaatga acatttggga ctcctggggc
     5401 catatataag agcagaagtt gaagataata tcatggtaac tttcagaaat caggcctctc
     5461 gtccctattc cttctattct agccttattt cttatgagga agatcagagg caaggagcag
     5521 aacctagaaa aaactttgtc aagcctaatg aaaccaaaac ttacttttgg aaagtgcaac
     5581 atcatatggc acccactaaa gatgagtttg actgcaaagc ctgggcttat ttctctgatg
     5641 ttgacctgga aaaagatgtg cactcaggcc tgattggacc ccttctggtc tgccacacta
     5701 acacactgaa ccctgctcat gggagacaag tgacagtaca ggaatttgct ctgtttttca
     5761 ccatctttga tgagaccaaa agctggtact tcactgaaaa tatggaaaga aactgcaggg
     5821 ctccctgcaa tatccagatg gaagatccca cttttaaaga gaattatcgc ttccatgcaa
     5881 tcaatggcta cataatggat acactacctg gcttagtaat ggctcaggat caaaggattc
     5941 gatggtatct gctcagcatg ggcagcaatg aaaacatcca ttctattcat ttcagtggac
     6001 atgtgttcac tgtacgaaaa aaagaggagt ataaaatggc actgtacaat ctctatccag
     6061 gtgtttttga gacagtggaa atgttaccat ccaaagctgg aatttggcgg gtggaatgcc
     6121 ttattggcga gcatctacat gctgggatga gcacactttt tctggtgtac agcaataagt
     6181 gtcagactcc cctgggaatg gcttctggac acattagaga ttttcagatt acagcttcag
     6241 gacaatatgg acagtgggcc ccaaagctgg ccagacttca ttattccgga tcaatcaatg
     6301 cctggagcac caaggagccc ttttcttgga tcaaggtgga tctgttggca ccaatgatta
     6361 ttcacggcat caagacccag ggtgcccgtc agaagttctc cagcctctac atctctcagt
     6421 ttatcatcat gtatagtctt gatgggaaga agtggcagac ttatcgagga aattccactg
     6481 gaaccttaat ggtcttcttt ggcaatgtgg attcatctgg gataaaacac aatattttta
     6541 accctccaat tattgctcga tacatccgtt tgcacccaac tcattatagc attcgcagca
     6601 ctcttcgcat ggagttgatg ggctgtgatt taaatagttg cagcatgcca ttgggaatgg
     6661 agagtaaagc aatatcagat gcacagatta ctgcttcatc ctactttacc aatatgtttg
     6721 ccacctggtc tccttcaaaa gctcgacttc acctccaagg gaggagtaat gcctggagac
     6781 ctcaggtgaa taatccaaaa gagtggctgc aagtggactt ccagaagaca atgaaagtca
     6841 caggagtaac tactcaggga gtaaaatctc tgcttaccag catgtatgtg aaggagttcc
     6901 tcatctccag cagtcaagat ggccatcagt ggactctctt ttttcagaat ggcaaagtaa
     6961 aggtttttca gggaaatcaa gactccttca cacctgtggt gaactctcta gacccaccgt
     7021 tactgactcg ctaccttcga attcaccccc agagttgggt gcaccagatt gccctgagga
     7081 tggaggttct gggctgcgag gcacaggacc tctactgagc ggccgctcga ggtcacccat
     7141 tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc
     7201 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7261 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7321 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7381 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7441 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7501 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7561 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7621 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     7681 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7741 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7801 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7861 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7921 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7981 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     8041 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8101 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8161 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     8221 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     8281 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     8341 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     8401 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     8461 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     8521 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     8581 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     8641 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     8701 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     8761 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     8821 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     8881 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8941 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9001 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     9061 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9241 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9661 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9901 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10081 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11161 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    11221 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
    11281 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    11341 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
    11401 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
    11461 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    11521 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    11581 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    11641 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
    11701 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    11761 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    11821 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    11881 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    11941 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    12001 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
    12061 actgcttact ggcttatcga aattaa
//